Bovine microvascular endothelial cells of separate morphology differ in growth and response to the action of interferon-γ

Five cell types recently isolated from the bovine corpus luteum differed in their epithelioid morphology and their cytoskeleton, but shared common criteria of microvascular endothelial cells^1,2. To give strong evidence for the separate entity, the growth rate of the 5 phenotypically different cells was studied. They were seeded at low density on day 0. Most of these cells were treated with 200 to 1000 U recombinant bovine interferon- (IFN-) for 3 days. The untreated remainder served as controls. Cell counts were made for all cultures on days 4, 7, 10 and 13. morphology: 13 d after treatment with IFN- senescent cells as well as intact cells occurred in cultures of cell types 1 to 4. Cultures of cell type 5 were apparently unchanged and resembled their untreated counterparts. Desminpositive cells in cultures of cell type 2 developed cell processes. Growth rate: In the absence of IFN-, the growth rate was high for cell types 3 and 4, moderate for cell type 1, and low for cell types 2 and 5. The presence of IFN- caused anti-proliferative effects. These were higher for cell types 3 and 4 than for cell types 1 and 2. IFN- could be cytotoxic on cell type 3. In contrast, the cytokine tended to support the cell growth of cell type 5. These findings substantiate the postulate that endothelial cells exhibiting separate morphology in culture also function differently.     

Presence of hsp65 in bacterial extracts (OM-89): A possible mediator of orally-induced tolerance?

Heat shock proteins (HSP) have been implicated in rodent models of autoimmunity, particularly arthritis, and there is suggestive though inconclusive evidence that they may also play a role in human autoimmune disease. The simplest hypothesis is based on molecular mimicry due to the amino-acid sequence homology between mammalian and microbial HSP. Recently OM-89, an extract of several strains ofEscherichia coli, has shown some efficacy in the treatment of rheumatoid arthritis (RA) when taken orally. Using species-specific antibodies, we show here that OM-89 contains the 65 kDa HSP (hsp65), while hsp65 was not detected in another bacterial extract containing other microorganisms, includingStaphylococcus aureus (OM-85). We suggest that if the human homologue of hsp65 is a relevant target antigen in the human disease, the efficacy of the preparation could be due to induction of oral tolerance or to switching the Th1 response towards Th2. Alternatively, even if the human hsp65 is not a target molecule in RA joints, OM-89 may evoke bystander suppression of joint inflammation via induction of TGF-secreting effector cells. These hypotheses should be tested in further studies.     

Occurrence of 3-epi-22-deoxy-20-hydroxyecdysone and its phosphoric ester in diapause eggs of the silkworm,Bombyx mori

Ecdysteroids in diapause eggs of the silkworm,Bombyx mori, were analyzed using high-performance liquid chromatography (HPLC) combined with radioimmunoassay (RIA). A relatively large amount of an unidentified free ecdysteroid and its phosphoric ester (conjugated form) were detected. These two compounds were isolated by a combination of column chromatography on silicic acid, thin-layer chromatography (TLC), and HPLC using a reverse-phase (RP) column. The purified compounds were identified as 3-epi-22-deoxy-20-hydroxyecdysone (22d20E) and 3-epi-22-deoxy-20-hydroxyecdysone 2-phosphate (22d20E2P) by means of mass spectrometry and nuclear magnetic resonance spectroscopy. to our knowledge, this is the first report of 22d20E and 22d20E2P.     

Fibronectin peptide DRVPHSRNSIT and fibronectin receptor peptide DLYYLMDL arrest gastrulation ofRana pipiens

Gastrulation is characterized by dramatic cell migration which is thought to require the interaction of cell adhesion molecules with extracellular molecules. We have tested two novel peptides, a fibronectin peptide and a fibronectin receptor peptide, for their effects on gastrulation of the leopard frogRana pipiens. The fibronectin peptide DRVPHSRNSIT corresponds to residues 1373–1383 of the cell-binding domain of fibronectin; the receptor peptide DLYYLMDL corresponds to residues 124–131 of 1 subunit of a variety of integrins including 51. Either of these peptides significantly inhibited gastrulation after being microinjected into mid-blastulae. These results indicate that these sequences may correspond to the ligand/receptor interaction sites of fibronectin and its receptor(s).     

信阳师院图书馆自动化管理系统实施方案分析

自动化管理是图书馆发展的必然趋势。本文借鉴有关高校图书馆自动化管理的经验教训,分析了以计算机管理为基础的自动化管理的三个阶段,从图书馆自动化系统应具备的功能出发,对系统模式、机型和软件进行了优化分析,最终确定了信阳师院图书馆自动化管理系统的实施方案。     

用Vitali复盖定义的非绝对积分

将一维空间Ｒ１上的非绝对积分推广到高维空间中;讨论了Ｂ积分的性质,并给出了Ｂ积分的Ｈａｒｎａｃｋ扩张定理．     

力学量算符本征函数的正交归一性

文中讨论了力学量算符本征函数的正交归一化性质,给出了得到正交归一化函数的方法。     

反胶束溶液和血红蛋白水溶液相平衡的研究

研究了十六烷基三甲基溴化铵－正庚烷反胶束溶液和牛血红蛋白水溶液的相平衡,探讨了有机相助表面活性剂种类和浓度、水相ｐＨ值、水相离子种类和强度、表面活性剂浓度的影响,适宜条件下,牛血红蛋白的萃取率可达９５％以上。对实验结果从表面活性剂极性头与蛋白质分子间的相互作用和反胶团包溶蛋白质时的空间阻碍作用上进行了解释。     

A continuous 200-ka palaeoclimatic record from stalagmite in Tangshan Cave, Nanjing

An oxygen isotope temperature record from 381 to 166 kaB. P. (²³⁰Th/²³⁴U dating and tuned ages) has been obtained by combining data on the isotopic composition of calcites with that of fluid inclusions trapped in a stalagmite from a limestone cave in which a fossil cranium deposited. Several laminae in microsequence of the stalagmite represent climatic events or shifts. The Nanjing man fossil was older than 381 kaB. P.